The Influence of the <i>FGF8</i> Gene on the Proliferation and Differentiation of Preadipocytes in Sheep
Han W., Zhang H., Gao F., Tian L., He Z., Wang G.
Laboratory Study, published in Animals (Basel) (2026) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Animals (Basel) (2026)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 41976100
- PMCID
- PMC13072408
- DOI
- 10.3390/ani16071121
Abstract (original English)
The growth and development of adipose tissue in sheep tails are closely associated with adipocyte proliferation and differentiation. However, the functional role and regulatory mechanisms of the FGF8 gene in sheep preadipocytes remain incompletely understood. In this study, liposome-mediated transfection was employed to overexpress the FGF8 gene and assess its effects on the proliferation and differentiation of sheep preadipocytes. The results of the Cell Counting Kit-8 (CCK-8) assay indicated that the overexpression of FGF8 promoted preadipocyte viability of preadipocytes. Subsequently, this was verified by RT-qPCR analysis, which showed significant upregulation of proliferation marker genes, including CyclinB ( p PCNA ) ( p CyclinD mRNA expression increased compared with the control group, though the increase was not statistically significant. During adipogenic induction, the mRNA expression levels of differentiation markers, such as Peroxisome Proliferator-Activated Receptor Gamma ( PPARγ ), CCAAT/Enhancer Binding Protein Alpha ( C/EBPα ), Adipocyte type Fatty Acid Binding Protein 4 ( FABP4 ), and Adiponectin , initially increased and then decreased. The expression of all four markers peaked on day 10 of induction, exceeding levels observed in the control group. In vitro experiments showed that FGF8 affected the proliferation and differentiation of sheep preadipocytes and ma
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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