Injectable Methylcellulose/Hyaluronic Acid/Collagen Hydrogel Loaded With Adipose-Derived Stem Cells Alleviates Skin Photoaging via Inhibition of HIF-1α: An in Vitro and in Vivo Study.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Background Adipose-derived stem cells (ADSCs) exhibit good anti-photoaging activity, yet inadequate homing numbers and low cell survival rates remain urgent challenges to overcome, and the underlying mechanisms are still unclear. Methods Methylcellulose (MC)/hyaluronic acid (HA)/Collagen I hydrogel (Gel) loaded with ADSCs (MHAC/ADSCs Gel) was prepared using the thermal dispersion method. Scanning electron microscopy was used to observe its microstructure, while swelling and degradation rates were determined by the pycnometer method. Subsequently, a photoaging HaCat cell model was established to evaluate the anti-photoaging potential of MHAC/ADSCs using the CCK-8 assay and Transwell experiment. Furthermore, qRT-PCR, WB, TargetScan bioinformatics analysis, and dual-luciferase reporter assays were conducted to investigate the underlying mechanisms of MHAC/ADSCs. Finally, a photoaging mouse model was established to assess the anti-photoaging activity of MHAC/ADSCs using H&E staining, Masson staining, immunohistochemistry, qRT-PCR, and WB. Results MHAC/ADSCs exhibited a 3D porous structure, remaining in a sol state at 20 ° C and forming a gel at 37 ° C. Additionally, it demonstrated excellent swelling properties and degradability. The results also revealed that MHAC/ADSCs showed significant anti-photoaging activity, promoting the migration of HaCat cells, increasing the expression o

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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