Intraoperative Optimization of Stromal Vascular Fraction for Remediation of Radiated Fracture Repair: Closing the Gap on Clinical Translation of Cell-Based Therapies.
Luby AO., Nelson JM., Sacks GN., Daniel M., Buchman L., Lynn JV.
Animal Study with a reported sample of 29, published in Ann Plast Surg (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Ann Plast Surg (2025)
- Country
- United States
- Reported sample size
- 29
- Source database
- PubMed
- PMID
- 40401910
- DOI
- 10.1097/SAP.0000000000004407
Abstract (original English)
Background Mechanical processing techniques to isolate the stromal vascular fraction (SVF) may optimize clinical translation of cell-based therapeutics. Therefore, the purpose of this study was to develop a technique for intraoperative isolation of SVF for immediate therapeutic use with the primary aim of enhancing bone healing at irradiated fracture sites. Methods Male Lewis rats (n = 29) were divided into groups: fracture, radiation with fracture, and radiation with fracture and SVF implantation. Experimental groups received 35 Gy of targeted radiation. All groups underwent mandibular osteotomy and external fixation. SVF was isolated from inguinal fat pads using Tulip Sizing Transfers, serial filtration, and centrifugation. The resultant cell pellet was implanted at the osteotomy site. After 40 days, bone union and mineralization were evaluated based on gross pathology and micro-computed tomography, respectively, and biomechanical strength testing was performed. Results SVF treatment increased union rates after radiation (79% vs 20%). Additionally, SVF improved both bone mineral density (666.2 ± 32.0 vs 312.2 ± 51.7; P = 0.000) and bone volume fraction (0.744 ± 0.072 vs 0.350 ± 0.041; P = 0.000) compared with the irradiated control. In fact, SVF treatment into irradiated fracture sites resulted in bone mineral density and bone volume fraction similar to the bone formed at non
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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