Intrinsic dynamics of the fat graft: in vitro interactions between the main cell actors.
Doornaert MAJ., Declercq H., Stillaert F., Depypere B., Van de Walle I., Cornelissen M.
Prospective Study, published in Plast Reconstr Surg (2012) — summary generated from the PubMed abstract.
Early human evidence such as case series or small samples is exploring possible benefits.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Prospective Study
- Journal
- Plast Reconstr Surg (2012)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 22777036
- DOI
- 10.1097/PRS.0b013e318267d3fb
- Citations
- 6
Abstract (original English)
Background Successful soft-tissue reconstruction requires autologous tissue transfer in respect to the increasingly important "replace like-with-like" principle. Autologous lipoaspirate material for fat grafting can easily be obtained in large amounts without substantial donor-site morbidity. The exact nature and fate of the different cells in the transplanted fat graft and their contribution to tissue reconstruction, however, remain largely unknown. Methods Adipose tissue was harvested from healthy female patients. CD34+ adipose-derived stem cells were isolated through magnetic-activated cell sorting and brought into co-culture with mature adipocytes in various culture medium conditions. Proliferation and differentiation of the adipose-derived stem cells were examined through histology, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays, and polymerase chain reaction assays. Results This study demonstrates that adipose-derived stem cells from fresh adipose tissue can be isolated within a few hours via magnetic-activated cell sorting with selection for CD34+ cells. All unpassaged adipose-derived stem cells in fresh adipose tissue are CD34+. Subsets include CD34+ CD31+ and CD34+ CD271+. No CD34+ CD45+ cells were present. Histological staining, polymerase chain reaction, and MTT assays confirm that purified mature adipose cells incite adipose-derived stem c
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
Evidence level
Early human evidence such as case series or small samples is exploring possible benefits.
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