Introducing human adipose-derived mesenchymal stem cells to Avance Ⓡ nerve grafts and NeuraGen Ⓡ nerve guides.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Background When direct nerve coaptation is impossible after peripheral nerve injury, autografts, processed allografts, or conduits are used to bridge the nerve gap. The purpose of this study was to examine if human adipose-derived Mesenchymal Stromal/Stem Cells (MSCs) could be introduced to commercially available nerve graft substitutes and to determine cell distribution and the seeding efficiency of a dynamic seeding strategy. Methods MTS assays examined the viability of human MSCs after introduction to the Avance Ⓡ Nerve Graft and the NeuraGen Ⓡ Nerve Guide. MSCs were dynamically seeded on nerve substitutes for either 6, 12, or 24 h. Cell counts, live/dead stains, Hoechst stains, and Scanning Electron Microscopy (SEM) revealed the seeding efficiency and the distribution of MSCs after seeding. Results The viability of MSCs was not affected by nerve substitutes. Dynamic seeding led to uniformly distributed MSCs over the surface of both nerve substitutes and revealed MSCs on the inner surface of the NeuraGen Ⓡ Nerve Guides. The maximal seeding efficiency of NeuraGen Ⓡ Nerve Guides (94%), obtained after 12 h was significantly higher than that of Avance Ⓡ Nerve Grafts (66%) (p = 0.010). Conclusion Human MSCs can be dynamically seeded on Avance Ⓡ Nerve Grafts and NeuraGen Ⓡ Nerve Guides. The optimal seeding duration was 12 h. MSCs were distributed in a uniform fashion on exposed su

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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