Isolation and characterization of equine native MSC populations.

Several human studies show positive signals, while research methods and sample sizes continue to develop.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Background In contrast to humans in which mesenchymal stem/stromal cell (MSC) therapies are still largely in the clinical trial phase, MSCs have been used therapeutically in horses for over 15 years, thus constituting a valuable preclinical model for humans. In human tissues, MSCs have been shown to originate from perivascular cells, namely pericytes and adventitial cells, which are identified by the presence of the cell surface markers CD146 and CD34, respectively. In contrast, the origin of MSCs in equine tissues has not been established, preventing the isolation and culture of defined cell populations in that species. Moreover, a comparison between perivascular CD146 + and CD34 + cell populations has not been performed in any species. Methods Immunohistochemistry was used to identify adventitial cells (CD34 + ) and pericytes (CD146 + ) and to determine their localization in relation to MSCs in equine tissues. Isolation of CD34 + (CD34 + /CD146 - /CD144 - /CD45 - ) and CD146 + (CD146 + /CD34 - /CD144 - /CD45 - ) cell fractions from equine adipose tissue was achieved by fluorescence-activated cell sorting. The isolated cell fractions were cultured and analyzed for the expression of MSC markers, using qPCR and flow cytometry, and for the ability to undergo trilineage differentiation. Angiogenic properties were analyzed in vivo using a chorioallantoic membrane (CAM) assay. Resul

Evidence level

Several human studies show positive signals, while research methods and sample sizes continue to develop.

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