Level D· Scientific groundwork from lab and animal studiesLaboratory StudyPubMed

Isolation, Culture, and Expansion of Mesenchymal Stem Cells.

Ferrin I., Beloqui I., Zabaleta L., Salcedo JM., Trigueros C., Martin AG.

Laboratory Study, published in Methods Mol Biol (2017) — summary generated from the PubMed abstract.

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Level D· Scientific groundwork from lab and animal studiesEvidence level of this study

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

  • Level A · Stronger Clinical Evidence
  • Level B · Emerging clinical evidence with positive signals
  • Level C · Early human research exploring benefits
  • Level D · Scientific groundwork from lab and animal studies
  • Emerging · Emerging topic under active research
Read the A–D evidence level guide

This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.

Study type
Laboratory Study
Journal
Methods Mol Biol (2017)
Country
United States
Reported sample size
—
Source database
PubMed
PMID
28353270
DOI
10.1007/978-1-4939-6921-0_13
Citations
23

Abstract (original English)

Mesenchymal stem cells (MSCs), together with hematopoietic stem cells (HSCs), are the most frequently used cell type for cell-based therapeutics. As for other cell types intended for research and translational use, it is important to establish correctly typed cell lines from human tissue donations. Here, we describe methods for isolating, culturing, and identifying MSCs from various tissues obtained through human tissue donation. The methods have been used in the context of a biobank, prepared as standard operating procedures (SOPs), ensuring traceability and reproducibility of cell production.

What this study does not prove

  • • This study does not prove SVF is an approved treatment or a replacement for standard care.
  • • This is preclinical work; animal or laboratory results cannot be applied to humans.

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

How we grade evidence
Biological Specimen BanksCell Culture TechniquesCells, CulturedCoculture TechniquesHematopoietic Stem CellsHumansMesenchymal Stem CellsReproducibility of Results

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