[Isolation, culture and identification of human adipose-derived mesenchymal stem cells].
Zhang WJ., Sun JJ.
Laboratory Study, published in Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi (2016) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi (2016)
- Country
- China
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 29771023
- DOI
- 10.13201/j.issn.1001-1781.2016.09.014
Abstract (original English)
Objective: To establish isolation and culture methods of human adipose derived mesenchymal stem cells(hADSCs) in vitro and to investigate the basic biological characteristics of hADSCs as seed cells involved in repair of epithelium during temporal bone surgery. Method: Human adipose tissue was collected after liposuction surgery from the cosmetic and plastic surgery center,and then was digested with 0.3% collagenase Ⅰ. hADSCs were isolated and adherent cultured. Multilineage differentiation potential of hADSCs were determined by differentiating cells into adipocytes and osteoblast. Phenotypes of cells were detected by flow cytometer, and cell growth curve was observed by MTT. Result: Obtained hADSCs were similar size, spindle-shaped fibroblasts-liked cells and had differentiation potential to adipocytes and osteoblast. Flow cytometry showed that cells were highly positive for CD105, CD90, CD29 and weakly positive for CD34 and CD45. MTT exhibited that hADSCs had a exponential growth phase. Conclusion: Isolated stem cells from human adipose tissue show the typical characterization of hADSCs, and can be used as seed cells involved in repair of epithelium during temporal bone surgery.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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