Isolation and differentiation of human mesenchymal stem cells obtained from second trimester amniotic fluid; experiments at Chang Gung Memorial Hospital.
Peng HH., Wang TH., Chao AS., Chang SD.
Laboratory Study, published in Chang Gung Med J (2007) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Chang Gung Med J (2007)
- Country
- China (Republic : 1949- )
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 18062170
Abstract (original English)
The aims of this study were to evaluate the efficacy of current techniques for isolating mesenchymal stem cells (MSCs) from amniotic fluid obtained by second-trimester amniocentesis as well as to determine their differentiation potential. We collected 50 samples of amniotic fluid by second-trimester amniocentesis. To obtain MSCs from amniotic fluid, the fluid was cultured using the two-stage culture protocol described in previous literature. Reverse transcription-polymerase chain reaction (RT-PCR) of a stem cell-specific transcription factor, octamer-binding protein 4 (Oct-4), was used to identify the characteristics of the MSCs cultured from amniotic fluid. Osteogeneic differentiation of these MSCs was confirmed by the presence of osteocalcin (a mineral-binding protein uniquely synthesized by osteoblasts) using RT-PCR and Von Kossa staining. Adipogenic differentiation of these MSCs was displayed by RT-PCR of adipocyte lipid-binding protein (a lipid-binding protein specifically in adipocytes) and Oil Red O staining. Amniotic fluid-derived MSCs were successfully isolated and cultured from six samples. These cells could express the pluripotent stem cell-specific transcription factor Oct-4 as confirmed by RT-PCR. Under specific culture conditions, amniotic fluid-derived MSCs could be successfully induced to differentiate into adipocytes and osteocytes, based on product analysis by
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
How we grade evidenceBrowse all related research
Filter the research library by this study's title keywords, author, or publication year.