[Isolation, expansion, and identification of the perivascular adipose-derived mesenchymal stem cells from rat aortas].

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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Abstract (original English)

Objective To establish a method for isolating and expanding perivascular adipose-derived mesenchymal stem cells (PV-ADSC) from rat aortas and to provide a reliable cellular model for investigating vascular remodeling and the related cardiovascular diseases. Methods Perivascular adipose tissue (PVAT) was aseptically collected from the aortas of 1-month-old Sprague Dawley (SD) rats, then rinsed, minced, digested, filtered, and seeded for culture. Upon reaching 80%-90% confluence, the cells were passaged for expansion, and their morphology and proliferation were continuously monitored. Passage 5 (P5) cells were harvested for flow cytometric analysis of surface CD marker expression, and directed differentiation assays with lineage-specific staining were performed to assess the trilineage (adipogenic, osteogenic, chondrogenic) differentiation potential. Results On day 4 of the primary culture, spindle-shaped, stellate, and polygonal cells were observed migrating from the explant edges. Between day 5 and 6, island-like cell clones emerged, and by day 7 to 8, the colonies had coalesced into a confluent monolayer with swirling or parallel alignment. After being passaged, the cells exhibited a homogeneous fibroblast-like spindle morphology and maintained robust proliferation, with no evident senescence or aberrant differentiation through P8. P5 cells showed high expression of the mesenc

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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