Isolation and growth of adipose tissue-derived stem cells.
Zachar V., Rasmussen JG., Fink T.
Laboratory Study, published in Methods Mol Biol (2011) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Methods Mol Biol (2011)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 21431509
- DOI
- 10.1007/978-1-60761-999-4_4
- Citations
- 34
Abstract (original English)
Fat tissue provides a rich and easily accessible supply of stem cells that feature the general differentiation potential and plasticity of mesenchymal stem cells. These stem cells, variably designated adipose-derived stem cells (ASCs), hold a great promise for regeneration in vivo and tissue engineering. In order to be able to obtain ASC preparations suitable for basic investigations as well as development of future therapeutic protocols, it is important that the critical isolation steps are properly carried out. Here, we describe in detail enzyme-based procedure for isolation and propagation of ASCs from the human subcutaneous fat tissue that is also adaptable to several animal species. The critical steps and impact of possible modifications on the final outcome are discussed, and useful hints are provided to streamline the troubleshooting.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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