Isolation, Primary Culture, and Differentiation of Preadipocytes from Mouse Brown Adipose Tissue.
Gao W., Kong X., Yang Q.
Animal Study, published in Methods Mol Biol (2017) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Methods Mol Biol (2017)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 28244035
- DOI
- 10.1007/978-1-4939-6820-6_1
Abstract (original English)
Evolutionally, brown adipose tissue (BAT) is developed for nonshivering thermogenesis to prevent hypothermia. BAT has a high capacity to dissipate chemical energy generated from metabolism of nutrients for heat production. Therefore when BAT is activated, nutrients are "burned' instead of being stored. This feature makes BAT an attractive target for obesity treatment. To investigate BAT function and regulation, brown adipocyte culturing is indispensable. This chapter describes a detailed protocol for isolation, primary culture, and differentiation of preadipocytes from mouse BAT. The preadipocytes can be used for investigating the regulation of brown fat cell differentiation. The differentiated brown adipocytes maintain major BAT features including high expression of uncoupling protein-1 and can be used to study BAT biology and pharmacology.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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