Label-free and real-time assessment of 660 nm red light photobiomodulation induced molecular alterations in human adipose derived mesenchymal stem cells using micro Raman spectroscopy.
Rastogi M., Chowdhury A., Chakraborty S., Sahu K., Majumder SK.
Laboratory Study, published in Spectrochim Acta A Mol Biomol Spectrosc (2024) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Spectrochim Acta A Mol Biomol Spectrosc (2024)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 39647267
- DOI
- 10.1016/j.saa.2024.125552
- Citations
- 1
Abstract (original English)
Therapeutic applications involving mesenchymal stem cells (MSCs) encounter challenges of attaining therapeutically potent and scaled up number during in-vitro batch culture. Recently, photobiomodulation (PBM) has emerged as a non-pharmacological method for enhancing MSC number, potency, and secretome production. However, the absence of a versatile, non-invasive technique to accurately identify PBM-induced biochemical alterations hinders the clinical translation of the approach. Raman spectroscopy (RS) can be a potential solution to this challenge. In this study, we demonstrate the utility of micro-RS to determine red light (∼660 nm) induced molecular alterations in human adipose tissue derived MSCs (hADMSCs) exposed to three different red light (∼660 nm) fluence; ∼3, 6 and 9 J/cm 2 . While the immediate changes in response to ∼660 nm exposure are subtle, at 6 and 24 h, there is increase in peak intensity of reducedCytochromes c, c1 and b, phenylalanine,CN stretching, CC lipids, OPO stretchingin cells. Maximum increase in intensity of these peaks was observed at ∼6 J/cm 2 . Raman peak at 1585 cm -1 , assigned to stretching vibration (CαCm) asymmetry of reduced Cyt c and sensitive to cellular redox status, shows notable change. Further, the intensity ratio of 1585 cm -1 and 1452 cm -1 , a suggestive Raman biomarker for cell proliferation, is increased in cells exposed to ∼3 & ∼6
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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