Lipid-associated macrophages are more abundant in subcutaneous than visceral adipose tissue in patients with obesity

Early human evidence such as case series or small samples is exploring possible benefits.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Objective Because white adipose tissue is infiltrated by several immune cells and their signature in individuals with obesity has not been fully characterized, we wanted to study the most abundant population, which is macrophages, a subtype of myeloid cell. Methods To address this objective, we performed transcriptomic analysis of subcutaneous adipose tissue (SAT)- and visceral adipose tissue (VAT)-infiltrated CD11b + myeloid cells from individuals with severe obesity. Results Our results showed that gene expression in human white adipose tissue-infiltrated CD11b + myeloid cells was depot-dependent. The expression of lipid-associated macrophage biomarkers was higher in SAT- than VAT-infiltrated CD11b + cells (TREM2, CD9, GPNMB, CD68). In contrast, VAT-infiltrated CD11b + cells overexpressed genes associated with a perivascular M2-like adipose tissue macrophage signature (LYVE1, TIMD4, MRC1). In addition, no classical gene expression polarization (M1 and M2) was shown when VAT and SAT CD11b + cells were compared. Finally, high levels of CD248, a sensor of lipids associated with insulin resistance, were found to be overexpressed in SAT- compared with VAT-infiltrated CD11b + myeloid cells. Conclusions This study characterizes for the first time the macrophage biomarker signature in human VAT- and SAT-infiltrated CD11b + myeloid cells from individuals with severe obesity. Further s

Evidence level

Early human evidence such as case series or small samples is exploring possible benefits.

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