Lipidomics Techniques Revealed the Adipogenic Differentiation Mechanism of Bovine Adipose-Derived Neural Crest Stem Cells.
Zhang K., Liang Z., Ding Y., Song X., Zhao R., Yan Y.
Laboratory Study, published in Animals (Basel) (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Animals (Basel) (2025)
- Country
- Switzerland
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 41227521
- PMCID
- PMC12606755
- DOI
- 10.3390/ani15213191
Abstract (original English)
Bovine adipose-derived neural crest stem cells (baNCSCs) are an ideal model for studying the mechanism of adipogenesis. Lipidomics provides a powerful technical means to comprehensively analyze the dynamic changes in lipid metabolism during cell differentiation. However, the lipidomic remodeling throughout the adipogenic differentiation of baNCSCs is still lacking in-depth research. This study used ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) to perform non-targeted lipidomic analysis on baNCSCs on day 0 (CON0) and day 9 (DIF9) of adipogenic induction and differentiation. Differential lipid metabolites were screened through multivariate statistical analysis and univariate analysis. A total of 1639 lipid molecules were identified. Compared with the CON0 group, 568 lipids were significantly altered in the DIF9 group, involving 6 major categories and 20 subclasses. The relative content and types of triacylglycerols (TAG) and diacylglycerols (DAG) increased significantly, becoming the most important markers of successful differentiation. Glycerophospholipids (GP) underwent complex remodeling, with subclasses such as phosphatidylethanolamine (PE), phosphatidylserine (PS), and cardiolipin (CL) significantly increased, indicating extensive restructuring of the cell and organelle membranes to adapt to lipid storage and energy metabolism. Sphingoli
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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