Lipoplasty: from body contouring to tissue engineering.
Ashjian PH., De Ugarte DA., Katz AJ., Hedrick MH.
Laboratory Study, published in Aesthet Surg J (2002) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Aesthet Surg J (2002)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 19331962
- DOI
- 10.1067/maj.2002.122940
- Citations
- 8
Abstract (original English)
Learning objectives The reader is presumed to have a broad understanding of plastic surgical procedures and concepts. After studying this article, the participant should be able to: Physicians may earn 1 hour of Category 1 CME credit by successfully completing the examination based on material covered in this article. The examination begins on page ***. Background The rapid development of disciplines such as cell therapy and tissue engineering has focused attention on stem cells as the ideal cellular substrate for new tissues. Human adipose tissue is a potential source of such stem cells. Objective We review the role of human adipose tissue in stem cell research and describe the procurement of stem cells from the stromal vascular fraction of human adipose tissue obtained through suction-assisted lipoplasty. Methods Raw lipoaspirate obtained through suction-assisted lipoplasty was washed in phosphate-buffered saline and digested with collagenase. The collagenase was then inactivated by fetal bovine serum and the cells were centrifuged for 10 minutes at 1200 x g. The resulting cell pellet was resuspended, plated, and maintained in nondifferentiating control media. Results Processing of 250 to 500 mL of suctioned tissue routinely yielded 2 to 6 x 10(8) processed lipoaspirate cells. Cell viability was typically >95%. These cells have been shown to differentiate in vitro into at lea
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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