Lithium ions (Li + ) and nanohydroxyapatite (nHAp) doped with Li + enhance expression of late osteogenic markers in adipose-derived stem cells. Potential theranostic application of nHAp doped with Li + and co-doped with
Alicka M., Sobierajska P., Kornicka K., Wiglusz RJ., Marycz K.
Laboratory Study, published in Mater Sci Eng C Mater Biol Appl (2019) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Mater Sci Eng C Mater Biol Appl (2019)
- Country
- Netherlands
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 30889661
- DOI
- 10.1016/j.msec.2019.02.073
- Citations
- 19
Abstract (original English)
Lithium (Li + ) ion due to its excellent bioactivity is one of the most well-studied element in bone-tissue engineering. In this study, we fabricated nanohydroxyapatite (nHAp) doped with Li + ions (5 mol% Li + :nHAp) and co-doped with lanthanide ions. We investigated the effects of nHAp, 5 mol% Li + :nHAp or Li + alone, on osteogenic differentiation of human Adipose Tissue-derived Stem Cells (hASCs), their proliferation, mitochondrial dynamics and apoptosis. Moreover, we monitored cell proliferation after treatment with samarium (III) (Sm 3+ ) and europium (III) (Eu 3+ ) ions co-doped 5 mol% Li + :nHAp as well as their luminescent property. The hASCs treated with 5 mol% Li + :nHAp and Li + ions proliferated more rapidly and differentiated effectively than control cells without undergoing apoptosis. Both, 5 mol% Li + :nHAp and Li + ions improved osteogenic differentiation of hASCs. Moreover they decreased expression of glycogen synthase kinase 3β (GSK3β) while increased β-catenin mRNA level. In addition, Li + , nHAp and 5 mol% Li + :nHAp improved mitochondrial dynamics and enhanced expression of neural differentiation marker genes. Collectively, the study indicates on pro-osteogenic and anti-apoptotic properties of nHAp doped with Li + and Li + alone. Moreover, unique properties of 5 mol% Li + :nHAp and 5 mol% Li + :nHAp co-doped with rare earth ions, such as Sm 3+ and Eu 3+ hav
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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