LLTH induces white adipose tissue browning via NF κB inhibition in ATM.
Lu Y., Feng J., Yan Y., Qiu J., Feng L.
Animal Study, published in Sci Rep (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Sci Rep (2025)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 41152352
- PMCID
- PMC12569381
- DOI
- 10.1038/s41598-025-21537-x
- Citations
- 2
Abstract (original English)
This study aimed to explore the role of macrophage polarization and TLR4/NF-κB pathway in promoting browning of white adipose tissue through different hypoxic exercises. (1) Fifty obese female rats were selected after 16 weeks of high-fat diet feeding and randomly divided into normoxic sedentary (NC), normoxic exercise (NE), hypoxic sedentary (HC), Living-High Training-High (HH), and Living-Low Training-High (LH) groups. Four weeks of hypoxic and exercise interventions were conducted to investigate changes in the expression of the TLR4/NF-κB pathway and inflammatory factors in obese rat adipose tissue and the expression of genes and proteins related to adipose tissue browning. (2) After the 4-week intervention, adipose tissue macrophages (ATM) were isolated from HH and LH groups, activated with NF-κB activator, and co-cultured with adipocyte-induced adipose-derived stem cells to explore the role of the TLR4/NF-κB pathway in promoting browning of white adipose tissue by inducing changes in macrophage polarization through different hypoxic exercise. (1) After 4 weeks of different hypoxic interventions, the proportion of M1 macrophages decreased (p < 0.01), and HH intervention increased the proportion of M2 macrophages (p < 0.05). The protein expression levels of TLR4, NF-κB, p-NF-κB, and MyD88 in ATM of the HH and LH groups were significantly lower than those of the NC group (p <
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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