Low Magnesium Concentration Enforces Bone Calcium Deposition Irrespective of 1,25-Dihydroxyvitamin D 3 Concentration
Rashid U., Becker SK., Sponder G., Trappe S., Sandhu MA., Aschenbach JR.
Animal Study, published in Int J Mol Sci (2023) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Int J Mol Sci (2023)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 37240030
- PMCID
- PMC10218185
- DOI
- 10.3390/ijms24108679
- Citations
- 3
Abstract (original English)
Efficient coordination between Mg 2+ and vitamin D maintains adequate Ca 2+ levels during lactation. This study explored the possible interaction between Mg 2+ (0.3, 0.8, and 3 mM) and 1,25-dihydroxyvitamin D 3 (1,25D; 0.05 and 5 nM) during osteogenesis using bovine mesenchymal stem cells. After 21 days, differentiated osteocytes were subjected to OsteoImage analysis, alkaline phosphatase (ALP) activity measurements, and immunocytochemistry of NT5E, ENG (endoglin), SP7 (osterix), SPP1 (osteopontin), and the BGLAP gene product osteocalcin. The mRNA expression of NT5E , THY1 , ENG , SP7 , BGLAP , CYP24A1 , VDR , SLC41A1 , SLC41A2 , SLC41A3 , TRPM6 , TRPM7 , and NIPA1 was also assessed. Reducing the Mg 2+ concentration in the medium increased the accumulation of mineral hydroxyapatite and ALP activity. There was no change in the immunocytochemical localization of stem cell markers. Expression of CYP24A1 was higher in all groups receiving 5 nM 1,25D. There were tendencies for higher mRNA abundance of THY1 , BGLAP , and NIPA1 in cells receiving 0.3 mM Mg 2+ and 5 nM 1,25D. In conclusion, low levels of Mg 2+ greatly enhanced the deposition of bone hydroxyapatite matrix. The effect of Mg 2+ was not modulated by 1,25D, although the expression of certain genes (including BGLAP ) tended to be increased by the combination of low Mg 2+ and high 1,25D concentrations.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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