Macroporous elastic cryogels based on platelet lysate and oxidized dextran as tissue engineering scaffold: In vitro and in vivo evaluations.
Şeker Ş., Elçin AE., Elçin YM.
Animal Study, published in Mater Sci Eng C Mater Biol Appl (2020) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Mater Sci Eng C Mater Biol Appl (2020)
- Country
- Netherlands
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 32204017
- DOI
- 10.1016/j.msec.2020.110703
- Citations
- 18
Abstract (original English)
In this study, three-dimensional macroporous cryogels were developed from platelet lysate (PL) and different concentrations of oxidized dextran (OD; 0.5, 1, 2, 4%). Subsequently, PL/OD scaffolds were characterized for potential use in tissue engineering applications. The pore size and morphology of the resulting cryogels were visualized using scanning electron microscopy (SEM). The pore size distributions were determined using mercury intrusion porosimetry (MIP). In vitro hydrolytic degradation, water uptake, mechanical properties and hemocompatibility were investigated. Extraction test was carried out to evaluate potential in vitro toxic effects of the PL/OD. The in vitro adhesion, proliferation, chondrogenic differentiation, and extracellular matrix production of human adipose stem cells (hASCs) on PL/OD cryogels were evaluated. In vivo biodegradation of the cryogels was investigated at the subcutaneous dorsal site of rats. SEM and MIP results indicated that PL/OD had a macroporous pore structure with pore sizes ranging between 10 and 200 μm. The cryogels completely degraded within 90-240 days post-implantation, depending on OD concentration. Histochemical analysis revealed high levels of cell and tissue infiltration into the pores of PL/OD. In vitro cytotoxicity findings indicated that the extracts of PL/OD 0.5 , PL/OD 1 and PL/OD 2 showed no cytotoxic effect, whereas that o
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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