Magnetic chitosan nanoparticle-exosome hydrogel enhances bladder function in diabetic bladder dysfunction via activating the FAK-p38 MAPK-GATA4 axis in adipose-derived mesenchymal stromal cells.
Zheng J., Zhang D., Li H., Jin R., Chen H., Sun X.
Animal Study, published in Regen Biomater (2026) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Regen Biomater (2026)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 41777717
- PMCID
- PMC12952527
- DOI
- 10.1093/rb/rbag007
Abstract (original English)
Previous attempts to combine adipose-derived mesenchymal stromal cells (ADSCs) with defocused low-energy shock wave (DLSW) have shown effectiveness in treating diabetic bladder dysfunction (DBD). However, the specific mechanisms underlying their therapeutic effects and strategies to enhance the colonization of ADSCs at the disease site remain challenging. Hereby, our investigation revealed that DLSW activated the FAK-p38 MAPK-GATA4 axis in ADSCs, resulting in enhanced secretion of vascular endothelial growth factor (VEGF) and nerve growth factor (NGF). Moreover, tube formation assay and major pelvic ganglia culture showed that the effects of VEGF and NGF on angiogenesis and nerve fiber growth were hindered by adding GATA4 inhibitors. We developed a thermosensitive hydrogel using chitosan nanoparticles (CSNP) incorporated with β-glycerophosphate and Fe 3 O 4 , and loaded it with exosomes (Exo) derived from DLSW-pretreated ADSCs to create a magnetic CSNP-Exo hydrogel. This hydrogel displayed favorable targeting ability, swelling properties, and sustained release profiles. In animal experiments, a DBD rat model was established by feeding a high-fat diet and injecting streptozotocin. Conscious cystometry results showed that the CSNP-Exo hydrogel ameliorated voiding function. Histological examinations revealed that CSNP-Exo hydrogel exhibited prolonged retention in the bladder and f
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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