Mechanical characterization of living and dead undifferentiated human adipose-derived stem cells by using atomic force microscopy.
Hu K., Zhao F., Wang Q.
Laboratory Study, published in Proc Inst Mech Eng H (2013) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Proc Inst Mech Eng H (2013)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 24044923
- DOI
- 10.1177/0954411913503064
- Citations
- 4
Abstract (original English)
In this article, to map the mechanical properties of undifferentiated human adipose-derived stem cells, local mechanical characterization is carried out on the adipose-derived stem cells. In addition, to distinguish the living and dead human adipose-derived stem cells, mechanical characterization is also implemented on both living and dead adipose-derived stem cells. In this study, Young's modulus of the cell membrane is used for representing the mechanical properties of cells. To obtain Young's modulus of cell membrane, the force-spectroscopy mode of atomic force microscopy is employed to measure the atomic force microscopy tip indentation depth and force on the cells. Then, Young's modulus is obtained through fitting these experimental data to the Hertzian contact mechanics model. The global Young's moduli of living and dead undifferentiated adipose-derived stem cells are about 1.27 and 18.61 kPa, respectively. This displays obvious gap of Young's modulus between the living and dead undifferentiated adipose-derived stem cells. Finally, comparison of the local Young's modulus shows deviation of the local Young's modulus for either living or dead undifferentiated adipose-derived stem cells, and the root-mean-square errors of the global Young's modulus of living and dead undifferentiated adipose-derived stem cells are about 0.48 and 5.05 kPa, respectively.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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