[Mechanism of platelet derived growth factor receptor alpha regulation of the bidirectional differentiation of glioma-associated oncogene homolog 1-positive mesenchymal stem cells in mice].
Zhang ZX., Xiao L., Wu LD., Yu CB., Mao J., Cao YG.
Laboratory Study, published in Zhonghua Kou Qiang Yi Xue Za Zhi (2023) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
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- Study type
- Laboratory Study
- Journal
- Zhonghua Kou Qiang Yi Xue Za Zhi (2023)
- Country
- China
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 37082846
- DOI
- 10.3760/cma.j.cn112144-20230206-00028
Abstract (original English)
Objective: To investigate the role of platelet derived growth factor receptor alpha (PDGFRα) on bidirectional differentiation of glioma-associated oncogene homolog 1-positive mesenchymal stem cells (Gli1 + -MSC). Methods: Breeding double reporter transgenic mice ROSA mT/mG /Gli1-Cre ERt2 /PDGFRα fl (experimental group) and ROSA mT/mG /Gli1-Cre ERt2 (control group), 20 mice in each of the two groups at four weeks of age were selected, MSC were isolated from the mouse aortic epithelium. After tamoxifen inducement, the two groups of Gli1 + -MSC were screened by green fluorescent protein (GFP) labeling and flow cytometry sorting. PDGFRα was conditionally knocked out in the experimental group, and the control group Gli1 + -MSC expressed PDGFRα normally. The two groups of Gli1 + -MSC were subjected to adipogenic induction and fibrogenic induction, the Western blotting was performed to detect PDGFRα, adipocyte markers [perilipin and CCAAT/enhancer binding protein alpha (C/EBPα)] and fibrogenic markers [alpha smooth muscle actin (α-SMA) and fibroblast-specific protein 1 (FSP-1)] and semi-quantitative analysis was performed. The degree of cellular adipose differentiation after bidirectional induction of Gli1 + -MSC in both groups was observed by oil red O staining and analyzed semi-quantitatively. Results: After tamoxifen induction, Gli1 + -MSC could be accurately isolated from flow cyt
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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