Level A· Stronger Clinical EvidenceSystematic ReviewEurope PMCOpen access

Medical hyperspectral imaging: an updated review of technology advancements and biomedical applications

Tran MH., Ma L., Yuan M., Fei B.

Systematic Review, published in J Biomed Opt (2026) — summary generated from the PubMed abstract.

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Level A· Stronger Clinical EvidenceEvidence level of this study

Relatively higher-quality human studies compared with other topics in this database, e.g. multiple RCTs or systematic reviews. This does not mean it is standard or approved care.

  • Level A · Stronger Clinical Evidence
  • Level B · Emerging clinical evidence with positive signals
  • Level C · Early human research exploring benefits
  • Level D · Scientific groundwork from lab and animal studies
  • Emerging · Emerging topic under active research
Read the A–D evidence level guide

This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.

Study type
Systematic Review
Journal
J Biomed Opt (2026)
Reported sample size
—
Source database
Europe PMC
PMID
41867475
PMCID
PMC13003176
DOI
10.1117/1.jbo.31.3.030901
Citations
2

Abstract (original English)

Significance Hyperspectral imaging (HSI) is an advanced spectral imaging technique that captures spatial and spectral information across numerous wavelength bands. This capability allows tissue characterization, disease detection and diagnosis, surgical guidance, and digital histopathology, making it an increasingly valuable tool with wide biological and medical applications. Aim We aim to provide readers with (1) an understanding of the principles and technological advancements in HSI, (2) a comprehensive overview of HSI data processing and analysis methods, and (3) an updated survey of biomedical applications, from disease detection, intraoperative imaging, to histopathology. Approach A systematic literature search was conducted using PubMed and Google Scholar with the keyword "hyperspectral imaging." We previously published a comprehensive review paper on medical HSI in 2014, which was widely cited in the field. Therefore, this updated review focused on new technology advancements and emerging applications. Based on their biological and medical relevance, 612 HSI papers were included and analyzed in this review. Results Recent advances in HSI span both hardware and computational techniques, including improvements in sensor technology, data processing and analysis, short-wave near-infrared imaging, and deep-learning and AI tools. HSI is actively explored for various applicati

What this study does not prove

  • • This study does not prove SVF is an approved treatment or a replacement for standard care.

Evidence level

Relatively higher-quality human studies compared with other topics in this database, e.g. multiple RCTs or systematic reviews. This does not mean it is standard or approved care.

How we grade evidence
AnimalsHumansImage Processing, Computer-AssistedHyperspectral Imaging

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