Melatonin Promotes Osteogenic Differentiation of Rat Adipose-Derived Stem Cells via the p38/MAPK Signaling Pathway.
Meng S., Han S., Kong M., Liu Z., Lin A., Qu C.
Animal Study, published in FASEB J (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- FASEB J (2025)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 40387393
- DOI
- 10.1096/fj.202403193RR
Abstract (original English)
While adipose-derived stem cells (ADSCs) transplantation represents an appealing therapeutic strategy for bone defect repair, the osteogenic capacity of ADSCs is largely limited. Melatonin has been demonstrated to contribute to the bone marrow stem cell (BMSC) osteogenesis. However, its effect on the osteogenic differentiation of ADSCs has not yet been determined. This study aims to identify whether melatonin exerts influences on the osteogenic differentiation in rat ADSCs. Rat ADSCs were isolated and identified. Subsequently, the impact of melatonin on the proliferation of rat ADSCs was examined. The effects of melatonin on the phenotypic features as well as marker genes and proteins of osteogenic differentiation were determined through the use of alkaline phosphatase (ALP) staining, ALP activity assay, alizarin red staining (ARS), RT-qPCR, western blot assay, and cellular immunofluorescence assay. To investigate the potential molecular mechanism through which melatonin promotes osteogenic differentiation of rat ADSCs, RNA sequencing, MAPK signaling pathway blocking assay and p38 mRNA interference assay were carried out. The results showed that melatonin at concentrations of 0-100 μM was safe and nontoxic for the proliferation of rat ADSCs, with the concentration at 100 μM exhibiting the most pronounced osteogenesis. Additionally, melatonin was observed to activate the p38/MAP
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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