Mesenchymal Progenitor Cells: Tissue Origin, Isolation and Culture.
Bourin P., Gadelorge M., Peyrafitte JA., Fleury-Cappellesso S., Gomez M., Rage C.
Laboratory Study, published in Transfus Med Hemother (2008) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Transfus Med Hemother (2008)
- Country
- Switzerland
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 21547114
- PMCID
- PMC3083284
- DOI
- 10.1159/000124734
- Citations
- 19
Abstract (original English)
SUMMARY: Since the pioneering work of Alexander Friedenstein on multipotent mesenchymal stromal cells (MSCs), a tremendous amount of work has been done to isolate, characterize and culture such cells. Assay of colony forming unit-fibroblasts (CFU-Fs), the hallmark of MSCs, is used to estimate their frequency in tissue. MSCs are adherent cells, so they are easy to isolate, and they show contact inhibition. Thus, several parameters must be taken into account for culture: cell density, number of passages, culture medium, and growth factors used. The purity of the initial material is not a limiting parameter. Similar but not identical cell populations are found in almost all mammal or human tissues. MSCs seem to be very abundant in adipose tissue but at low frequency in blood from umbilical cord or in adult tissue. The culture conditions are very similar, whatever the source of cells. Because of their favorable properties, MSCs are very promising tools for regenerative medicine. Seit der bahnbrechenden Arbeit von Alexander Friedenstein zu multipotenten mesenchymalen Stammzellen (MSCs) wurde enorm viel Zeit und Arbeit in die Isolierung, Charakterisierung und Kultivierung dieser Zellen investiert. Zur Bestimmung ihrer Häufigkeit in Gewebe wird der CFU-Fs (colony forming unit-fibroblasts)-Assay, das Markenzeichen von MSCs, verwendet. Da MSCs ad-härente Zellen sind, sind sie leicht zu
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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