Mesenchymal Stem Cell Therapy Fails to Improve Fat Graft Retention: Cryopreserved and Cultured Syngeneic and Allogeneic Mesenchymal Stem Cells in a Rat Model.
Alstrup T., Pedersen JO., Kjær EM., Poulsen KJ., Steinfurth S., Pedersen M.
Animal Study, published in Plast Reconstr Surg (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
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- Study type
- Animal Study
- Journal
- Plast Reconstr Surg (2025)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 40178539
- DOI
- 10.1097/PRS.0000000000012121
Abstract (original English)
Background Fat grafting is a gentle technique used to correct soft-tissue defects, but it is challenged by low graft survival rates. To enhance retention, mesenchymal stromal cells (MSCs) have been explored. Autologous MSC therapy has shown promise, but comes with logistical and cost challenges. Allogeneic MSC therapy offers a more feasible solution, with preclinical studies suggesting improved fat graft retention from allogenic MSC therapy. This study aimed to expand the preclinical research by investigating the use of cryopreserved allogeneic MSCs. Methods Using an immunocompetent rat model for autologous fat grafting, the effect of allogeneic MSCs on 3-month fat graft retention was investigated. In a series of experiments, the effect of dosing (0.2 × 10 6 to 25 × 10 6 MSCs/mL), allogenicity (allogeneic and syngeneic), and cryopreservation was assessed. Results The authors' findings did not indicate any beneficial effect from cryopreserved allogeneic MSC therapy for fat grafting across the tested concentrations. In fact, increasing the dosage resulted in lower fat graft retention, reduced expression of adipose markers, and increased fibrosis. Administration of cryopreserved syngeneic MSCs had no beneficial effect on long-term fat graft retention either. Substituting cryopreserved MSCs with freshly harvested MSCs also did not enhance fat graft retention. Conclusions Unlike pre
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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