Mesenchymal Stem Cells Early Response to Low-Dose Ionizing Radiation.
Konkova M., Abramova M., Kalianov A., Ershova E., Dolgikh O., Umriukhin P.
Laboratory Study, published in Front Cell Dev Biol (2020) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Front Cell Dev Biol (2020)
- Country
- Switzerland
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 33381502
- DOI
- 10.3389/fcell.2020.584497
Abstract (original English)
Mesenchymal stem cells (MSCs) are applied as the therapeutic agents, e.g., in the tumor radiation therapy. To evaluate the human adipose MSC early response to low-dose ionizing radiation (LDIR). We investigated different LDIR (3, 10, and 50 cGy) effects on reactive oxygen species production, DNA oxidation (marker 8-oxodG), and DNA breaks (marker ɣ H2AX) in the two lines of human adipose MSC. Using reverse transcriptase-polymerase chain reaction, fluorescence-activated cell sorting, and fluorescence microscopy, we determined expression of genes involved in the oxidative stress development ( NOX4 ), antioxidative response ( NRF2 ), antiapoptotic and proapoptotic response ( BCL2 , BCL2A1 , BCL2L1 , BIRC2 , BIRC3 , and BAX1 ), in the development of the nuclear DNA damage response (DDR) ( BRCA1 , BRCA2 , ATM , and P53 ). Cell cycle changes were investigated by genes transcription changes ( CCND1 , CDKN2A , and CDKN1A ) and using proliferation markers KI-67 and proliferating cell nuclear antigen (PCNA). Fifteen to 120 min after exposure to LDIR in MSCs, transient oxidative stress and apoptosis of the most damaged cells against the background of the cell cycle arrest were induced. Simultaneously, DDR and an antiapoptotic response were found in other cells of the population. The 10-cGy dose causes the strongest and fastest DDR following cell nuclei DNA damage. The 3-cGy dose induces a
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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