[Metabolic dysregulation in adipose tissue macrophages and its link to obesity and obesity with type 2 diabetes based on single-cell transcriptome sequencing].

Early human evidence such as case series or small samples is exploring possible benefits.

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• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Objective: To identify the association between metabolic and functional dysregulation of adipose macrophages in obesity and type 2 diabetes. Methods: Major cell types of adipose tissue were dissected with canonical markers from the single-cell sequencing dataset HRA002549, which profiled the stromal vascular fraction from 2 healthy controls (2 females), 3 obese patients (1 female), and 3 obese patients with type 2 diabetes (1 female). Subtypes of macrophages were then discerned by unsupervised clustering and functional annotation. Single-cell level metabolic activities were profiled with a flux-balance algorithm. The alternation of metabolic activity in obesity was identified with metabolic differential and enrichment analysis. The associations between metabolic and functional alternation were identified with Spearman's correlation tests. Results: A total of four subpopulations of adipose tissue macrophages with different functional characteristics were identified. The distribution of each macrophage subpopulation in adipose tissue differed between the healthy control group, the obese group, and the obese group with type 2 diabetes (all P 0.05). Differential analysis identified the activation of pro-inflammatory and metabolic-sensing pathways including phosphatidylinositol 3-kinase/protein kinase B (PI3K-Akt), Janus kinase/signal transducer and activator of transcription (JAK-S

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Early human evidence such as case series or small samples is exploring possible benefits.

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