METTL3-Mediated lncSNHG7 m 6 A Modification in the Osteogenic/Odontogenic Differentiation of Human Dental Stem Cells
Yang Y., Zeng J., Jiang C., Chen J., Song C., Chen M.
Laboratory Study, published in J Clin Med (2022) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- J Clin Med (2022)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 36614914
- PMCID
- PMC9821659
- DOI
- 10.3390/jcm12010113
- Citations
- 10
Abstract (original English)
Background : Human dental pulp stem cells (hDPSCs) play an important role in endodontic regeneration. N6-methyladenosine (m 6 A) is the most common RNA modification, and noncoding RNAs have also been demonstrated to have regulatory roles in the expression of m 6 A regulatory proteins. However, the study on m 6 A modification in hDPSCs has not yet been conducted. Methods : Single base site PCR (MazF) was used to detect the m 6 A modification site of lncSNHG7 before and after mineralization of hDPSCs to screen the target m 6 A modification protein, and bioinformatics analysis was used to analyze the related pathways rich in lncSNHG7. After knockdown and overexpression of lncSNHG7 and METTL3, the osteogenic/odontogenic ability was detected. After METTL3 knockdown, the m 6 A modification level and its expression of lncSNHG7 were detected by MazF, and their binding was confirmed. Finally, the effects of lncSNHG7 and METTL3 on the Wnt/β-catenin pathway were detected. Results : MazF experiments revealed that lncSNHG7 had a m 6 A modification before and after mineralization of hDPSCs, and the occurrence site was 2081. METTL3 was most significantly upregulated after mineralization of hDPSCs. Knockdown/ overexpression of lncSNHG7 and METTL3 inhibited/promoted the osteogenic/odontogenic differentiation of hDPSCs. The m 6 A modification and expression of lncSNHG7 were both regulated by MET
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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