SVF
Level D· Preclinical EvidenceAnimal Study

Microenvironment-directed chondrogenesis of adipose-derived mesenchymal stem cells in hyaline, elastic, and fibrocartilage in vivo.

Han Y., Ma H., Chen J., Cheng L., Li K., Zhang B.

Animal Study on Cartilage Damage, Meniscus Injury, published in Cell Tissue Bank (2026) — summary generated from the PubMed abstract.

This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.

Study type
Animal Study
Journal
Cell Tissue Bank (2026)
Country
Netherlands
Reported sample size
—
PMID
41824109
DOI
10.1007/s10561-026-10219-1

Abstract (original English)

Cartilage exhibits remarkable subtype diversity-hyaline, fibrocartilage, and elastic-each with unique extracellular matrix architecture and mechanical function. Despite the clinical promise of adipose-derived mesenchymal stem cells (ADMSCs) for cartilage regeneration, whether distinct in vivo cartilage microenvironments can instruct ADMSC differentiation toward corresponding subtypes remains poorly defined. Green fluorescent protein-labeled human ADMSCs were encapsulated in a fibrin hydrogel and implanted into three representative cartilage sites-auricular (elastic), articular (hyaline), and meniscal (fibrocartilage)-in an immunodeficient rat model. Regenerated tissues were harvested at 4 weeks for histology (H&E), immunofluorescence analysis of hyaline (COL II, aggrecan), fibrocartilage (COL I, tenomodulin), and elastic (fibrillin-1, elastin) markers, and RT-PCR quantification of lineage-associated gene expression. ADMSCs survived and engrafted within all three microenvironments. Articular cartilage implants exhibited strong hyaline-like differentiation, characterized by intense COL II and aggrecan expression and upregulation of SOX9, COL2A1, and ACAN. Meniscal implants instead displayed a fibrocartilage-like profile with robust COL I and tenomodulin expression and elevated COL1A1, TNMD, and SCX transcripts. In contrast, auricular cartilage implants showed negligible expressio

What this study does not prove

  • • This study does not prove SVF is an approved treatment or a replacement for standard care.
  • • This is preclinical work; animal or laboratory results cannot be applied to humans.

Evidence level

Evidence comes from animal or laboratory studies and has not been confirmed in humans.

How we grade evidence
AnimalsMesenchymal Stem CellsChondrogenesisHumansAdipose TissueFibrocartilageRatsHyaline CartilageCell DifferentiationCellular Microenvironment
KneeCartilage DamageMeniscus Injury
View on PubMedDOI

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