miR-19a-3p and miR-19b-3p repress Nurr1 and Nur77 to promote microglial inflammation after spinal cord injury

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Background Spinal cord injury (SCI)-induced neuropathic pain affects up to 60% of individuals with SCI and is closely linked to microglia-driven neuroinflammation. Neuroinflammatory processes after SCI are major contributors to the development and persistence of chronic pain. MicroRNAs (miRNAs) have emerged as regulators of neuroinflammation. There are higher levels of circulating miR-19a and miR-19b in persons living with SCI with neuropathic pain compared to those with no pain. These miRNAs are associated with altered the neuroprotective genes Nurr1 and Nur77. Methods Primary microglia cultures and a rat spinal cord injury model were used to investigate the regulatory effects of miR-19a and miR-19b on Nurr1 and Nur77 expression. Results Our study shows that miR-19a and miR-19b and their binding sites in Nurr1's 3' UTR are highly conserved across vertebrates, suggesting functional importance. Through in vitro microglia cultures and in vivo rat SCI models, we demonstrate that these miRNAs negatively regulate Nurr1, Nur77, and inflammatory gene expression. Protein-protein interaction network analysis highlights transcription factors such as MYC, RUNX1, and STAT3 as central to this regulatory network. Conclusion These findings support a model in which miR-19a and miR-19b contribute to microglia-driven neuroinflammation after SCI and highlight their potential as therapeutic target

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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