miR-200a-3p in Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Attenuates UVB-Induced Skin Inflammatory Response and Oxidative Stress via Keap1-Nrf2 Pathway

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Ultraviolet (UV) radiation induces skin damage primarily through oxidative stress and excessive inflammation. Exosomes derived from mesenchymal stem cells have emerged as promising therapeutic agents for tissue repair. Here, we investigated the protective effects of human umbilical cord mesenchymal stem cell-derived exosomes (HuMSC-Exos) on UVB-induced skin injury in HaCaTs and C57BL/6 mice. HuMSC-Exos significantly reduced reactive oxygen species (ROS) levels, suppressed proinflammatory cytokines (IL-1β, TNF-α, and IL-6), and improved cell migration. Mechanistically, HuMSC-Exos inhibited Keap1, enhanced both total and phosphorylated Nrf2 expression, promoted its nuclear translocation, and upregulated antioxidant genes ( HMOX1 , NQO1 , CAT , and SOD2 ). miR-200a-3p in HuMSC-Exos mediated these effects by targeting Keap1. Furthermore, preliminary data suggested that HuMSC-Exos also attenuate inflammatory responses via the NF-κB pathway. In vivo, HuMSC-Exos attenuated UVB-induced skin injury and inflammation by activating the Nrf2 signaling cascade. Collectively, our findings reveal a novel protective mechanism and highlight the therapeutic potential of HuMSC-Exos in mitigating UV-induced skin damage by modulating oxidative stress and inflammation.

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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