MiR-24-3p delivered by adipose derived stem cells exosomes alleviate macrophage pyroptosis via NLRP3/Caspase1/GSDMD in sepsis ALI.
Shen K., Wang P., Zhang H., Wang X., Fu H., Li J.
Animal Study, published in Stem Cell Res Ther (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Stem Cell Res Ther (2025)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 41146344
- PMCID
- PMC12560335
- DOI
- 10.1186/s13287-025-04722-7
- Citations
- 3
Abstract (original English)
Background Sepsis is a life-threatening condition associated with high morbidity and mortality, frequently resulting in acute lung injury (ALI) as one of its most severe complications. The pathogenesis of sepsis-induced ALI is significantly influenced by excessive inflammation, particularly that mediated by macrophages, plays a critical role in the pathogenesis of sepsis-induced ALI. This study sought to explore the protective effects and underlying mechanisms of adipose-derived stem cell exosomes (ADSCs-exo) on macrophage-mediated inflammation and pyroptosis. Methods ADSCs-exo were isolated and characterized using nanoparticle tracking analysis (NTA), Western blotting, and transmission electron microscopy. A macrophage pyroptosis model was established through lipopolysaccharide (LPS) stimulation. The effects of ADSCs-exo on the secretion of inflammatory cytokine and pyroptosis-related markers (NLRP3, Caspase1, GSDMD) was evaluated. Mechanistic studies included dual-luciferase reporter assays and NLRP3 inhibition experiments. In vivo, a cecal ligation and puncture (CLP) sepsis model was utilized to evaluate the impact of ADSCs-exo on pulmonary inflammation, histopathological damage, survival rates, macrophage polarization (M1/M2), and components of the pyroptosis pathway (NLRP3, Caspase1). Results Isolated ADSCs-exo displayed characteristic features of exosomes. The administrat
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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