miR-26a-5p inhibits the proliferation and myogenic differentiation of chicken BMSCs by targeting MDFIC.
Liu S., Lu Y., Wu L., Qin Z., Zhao C., Zhou Z.
Laboratory Study, published in Poult Sci (2026) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Poult Sci (2026)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 42335781
- DOI
- 10.1016/j.psj.2026.107181
Abstract (original English)
Coordinated development and homeostasis among bone, muscle, and adipose tissue are essential for the sustainable advancement of the broiler industry. Bone marrow mesenchymal stem cells (BMSCs) possess multi-lineage differentiation potential and provide an in vitro model for investigating regulatory mechanisms of the "bone-muscle-adipose" balance. Nevertheless, microRNA (miRNA)-mRNA regulatory network and the functions of key miRNAs during myogenic differentiation of chicken BMSCs remain unclear. We profiled mRNA and miRNA expression during this process to identify key miRNAs regulating BMSC proliferation and differentiation. mRNA sequencing (mRNA-seq) and miRNA sequencing (miRNA-seq) before and after 5-azacytidine (5-Aza) induction identified 2,233 differentially expressed genes (DEGs) and 67 differentially expressed miRNAs (DE miRNAs). DEGs were mainly enriched in cell cycle and DNA replication pathways, whereas predicted targets of DE miRNAs were predominantly enriched in calcium signaling pathway. A candidate miRNA-mRNA regulatory network was constructed using 13 skeletal muscle-related DE miRNAs and inversely expressed target mRNAs, with target genes enriched in protein processing in the endoplasmic reticulum and Wnt signaling pathways. RNAhybrid prediction and dual-luciferase reporter assays confirmed that miR-26a-5p directly targets MyoD Family Inhibitor Domain Containing
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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