MiR-33a inhibits the adipogenic differentiation of ovine adipose-derived stromal vascular fraction cells by targeting SIRT6.
Wang Q., Pan Y., Zhao B., Qiao L., Liu J., Liang Y.
Animal Study, published in Domest Anim Endocrinol (2020) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Domest Anim Endocrinol (2020)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 32653737
- DOI
- 10.1016/j.domaniend.2020.106513
- Citations
- 5
Abstract (original English)
Adipose tissue is important for the regulation of energy balance through its metabolic, cellular, and endocrine functions. Furthermore, the excessive storage of subcutaneous fat can seriously affect the health and carcass traits of domestic animals. Stromal vascular fraction (SVF) cell adipogenic differentiation increases the number of differentiated adipocytes and plays a role in lipid deposition. The adipogenic differentiation of SVF cells is regulated by various factors, including microRNAs and cytokines. Sirt6 and miR-33a are known to be involved in metabolism and adipogenesis, respectively; however, their effects on the adipogenic differentiation of ovine SVF cells were previously unknown. Thus, the aim of this study was to investigate this. The results showed that SIRT6 is a binding target for miR-33a. Moreover, overexpression or inhibition of miR-33a was found to change the expression of SIRT6 messenger RNA and protein. Furthermore, modulating SIRT6 altered the expression of adipogenic marker genes. In addition, miR-33a and SIRT6 were found to play opposing roles in adipogenesis. Specifically, we demonstrated that miR-33a is involved in the negative regulation of ovine SVF cell adipogenic differentiation by inhibiting the expression of SIRT6. These findings reveal a key role for miR-33a and SIRT6 in adipogenesis, which will enrich our understanding of the regulatory fact
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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