miR-9-5p alleviates the development of abdominal aortic aneurysm by regulating the differentiation of CD4⁺IL-10⁺T cells via targeting the crosstalk between Nrf2 and NF-κB signaling pathways

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Background Abdominal aortic aneurysm (AAA), a gradual segmental dilatation of the abdominal aorta, is associated with a high mortality rate. The pathophysiological molecular mechanisms underlying AAA remain unclear. In recent years, changes in miRNA levels have been reported to be involved in the development and treatment of AAA. This study aimed to investigate the potential targets and underlying mechanisms of miR-9-5p in attenuating AAA progression by modulating the inflammatory response. Materials and methods Biochemical kits were used to measure the levels of inflammatory factors, antioxidant enzyme activity, and serum oxidative stress in normal and AAA model mice. miR-9-5p overexpression was achieved by transfecting miR-9-5p mimics into CD4 + T cells and administering an miR-9-5p agomir to the mice. The effect of miR-9-5p overexpression was evaluated by detecting the expression level of miR-9-5p in CD4 + T cells through qRT-PCR. The NF-κB/Nrf2 pathway levels were assessed using immunofluorescence, western blotting, and quantitative PCR. miR-9-5p expression was modulated by transfecting either miR-9-5p mimics or inhibitors, and the impact on CD4 + IL-10 + T-cell differentiation was analyzed using flow cytometry. Results Compared with that in the control group, miR-9-5p expression in CD4 + T cells from the peripheral blood of AAA model mice was decreased by 28%. In vivo, miR

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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