Mitochondrial Abundant Heat Soluble (MAHS) Protein Expression Modulates Metabolic Dynamics in Human Adipose-Derived Stem Cells.
Rolsma JL., Li J., Soh R., Nieh C., Yeh B., Darch W.
Laboratory Study, published in Int J Mol Sci (2026) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Int J Mol Sci (2026)
- Country
- Switzerland
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 42511631
- DOI
- 10.3390/ijms27146289
Abstract (original English)
Adipose-derived stem cells (ASCs) possess substantial regenerative potential, with lineage fate tightly coupled to metabolic dynamics. In prior work, we found that expression of the tardigrade-derived protein Mitochondrial Abundant Heat Soluble (MAHS) increased stress tolerance and promoted ASC osteogenic differentiation over adipogenic differentiation, although the mechanism remained unclear. Here, we leverage transcriptomic and functional assays to investigate how MAHS expression influences ASC metabolism. Transcriptomic profiling revealed incomplete adipogenic activation alongside upregulation of ossification-associated genes (e.g., SERPINF1 ), as well as broader changes in metabolic, Wnt, and YAP signaling pathways consistent with a shift toward osteogenic programming. Functionally, MAHS-expressing ASCs exhibited increased matrix calcium deposition in osteogenic culture, as well as decreased ATP and cAMP levels during basal culture. Further, treatment with forskolin increased cAMP levels, while forskolin or Compound C increased lipid accumulation in MAHS-expressing ASCs. Together, these findings suggest that MAHS expression perturbs metabolic dynamics and signaling networks in ASCs and may contribute to impaired adipogenesis and reinforcement of osteogenic bias. More broadly, this work highlights potential regulatory points that could be targeted to restore lineage balance
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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