A modified technique for mechanical isolation of stromal vascular fraction yields increased final product volume and high viable nucleated cells count.

Early human evidence such as case series or small samples is exploring possible benefits.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Purpose Purpose of the present study is to report a modified protocol for stromal vascular fraction (SVF) isolation from abdominal fat, analyse its cellular composition and gene expression profile, and verify the safety and feasibility of the harvesting-preparation technique and subsequent intra-articular knee injection. Methods The SVF was obtained after mechanical dissociation of the autologous harvested adipose tissue. It was combined with autologous platelet-rich plasma and subsequently intra-articularly injected into both knees of patients with osteoarthritis. Part of the SVF solution was used to analyse its cellular composition via flow cytometry and its gene expression profile via real-time polymerase chain reaction. Any postinjection complications were documented. Results Twenty-three patients (10 female; 62.4 ± 8.4 years old) were enrolled. Posttreatment adverse events were mild and spontaneously resolved. Patients needed 3.7 ± 1.3 days for their knee to feel the same as before the injection. No patient provided less than 60 mL of lipoaspirate. Per 1 mL of SVF the total amount of viable nucleated cells was 49.7 ± 22.9 × 10 6 on average, including 44.5 ± 23.2 × 10 6 CD90+/CD105+ adipose-derived stem cells, 0.54 ± 0.18 × 10 6 hematopoietic stem cells, 2.71 ± 1.18 × 10 6 pericytes and 1.88 ± 0.64 × 10 6 endothelial cells. The polymerase chain reaction analysis revealed th

Evidence level

Early human evidence such as case series or small samples is exploring possible benefits.

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