Monitoring ADSC Metabolism Using the Seahorse Analyzer.
Rittenhouse D., Zwezdaryk KJ.
Animal Study, published in Methods Mol Biol (2024) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Methods Mol Biol (2024)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 38478245
- DOI
- 10.1007/978-1-0716-3762-3_24
Abstract (original English)
Bioenergetic and biosynthetic processes are key indicators regulating adipose-derived stromal/stem cell (ADSC) function, health, and differentiation. A common method used to metabolically profile cells is the Seahorse XF Analyzer. This live-cell assay can be used to define key metabolic pathways, including glycolysis and oxidative phosphorylation. Here, we share optimized protocols to characterize metabolism of ADSCs under basal conditions and provide insight into further assays defining metabolic changes and/or dependency during ADSC differentiation.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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