A Multichamber Gas System to Examine the Effect of Multiple Oxygen Conditions on Cell Culture.
Khoury S., Haj Khalil T., Palzur E., Srouji S.
Laboratory Study, published in Tissue Eng Part C Methods (2021) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Tissue Eng Part C Methods (2021)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 33353455
- DOI
- 10.1089/ten.TEC.2020.0288
- Citations
- 2
Abstract (original English)
The classic bone tissue engineering model for bone regeneration combines three elements: scaffolds, biomaterials, and mesenchymal stem cells (MSCs). Incorporation of MSCs and growth factors into a scaffold implanted into the area of bone injury is a proven strategy to achieve successful bone regeneration as demonstrated in the literature. However, a major limitation of using bone grafts or scaffolds is oxygen (O 2 ) deprivation in the inner sections of the construct, due to lack of adequate vascularization. To address this limitation, we proposed two treatment strategies for MSC-seeded constructs or adipose tissue scaffolds before implantation: (1) O 2 enrichment and (2) acclimation to hypoxia. Based on previous studies, the significance of the different O 2 concentrations on MSC biological characteristics remains controversial. Therefore, the optimal O 2 condition for engineered bone tissues should be determined. Thus, we designed an innovative multichamber gas system aimed to simultaneously assess the effects of different O 2 levels on cell culture. This system was assembled using three isolated chambers integrated into a single incubator. To explore the efficacy of our method, we investigated the effect of hyperoxia, normoxia, and hypoxia, (50-60%, 21%, and 5-7.5% O 2 , respectively) on the biological characteristics of human adipose-derived MSCs: immunophenotyping, adhesion
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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