Myoblast and ADSC coculture on conductive highly aligned nanofiber scaffolds for human skeletal muscle tissue engineering.
Shi X., Cai A., Arkudas A., Horch RE., Jabeen S., Schubert DW.
Laboratory Study, published in Biomed Mater (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Biomed Mater (2025)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 41192076
- DOI
- 10.1088/1748-605X/ae1c08
Abstract (original English)
Conductive materials play a crucial role in enhancing functional performance in muscle tissue engineering. This study investigates the impact of the conductive polymer polyaniline (PANi) in Polycaprolactone (PCL)-collagen Type I (PCL-collagen I) nanofiber scaffolds designed to support the coculture of human adipose-derived stem cells (ADSCs) and myoblasts (Mbs). The effect of varying PANi concentrations (0%, 2%, 4%, 6%) in PCL-collagen I nanofiber scaffolds was evaluated concerning the cell alignment, differentiation and gene expression of cocultured Mbs and ADSC. Nanofiber scaffolds with different PANi concentrations were analyzed. Acetic acid was used as a non-toxic and biocompatible solvent for electrospinning the nanofibers. In vitro experiments involved a 1:1 coculture of Mbs and ADSCs for up to 28 d on the scaffolds. The cell viability, differentiation and myotube morphology were assessed using live-dead-assay, CCK-8-assay, immunofluorescence staining and gene expression analysis. Scaffolds with 2% and 4% PANi showed a higher percentage of live cells compared to the control at both 7 and 28 d. The nanofibers with 2%, 4% and 6% PANi concentration showed promising results in terms of cell differentiation and myotube morphology. After 14 d, the scaffolds with 4% PANi showed superior cell differentiation with strong myotube alignment along the nanofibers. At higher PANi conce
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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