MYSM1 regulates the proliferation and differentiation of bovine skeletal muscle satellite cells via BRG1-mediated activation of the AKT/mTOR/NF-κB signaling pathway
Zhang C., Li Y., Zhang W., Ma T., Li X., Guo Y.
Laboratory Study, published in Anim Biosci (2026) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Anim Biosci (2026)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 41414839
- PMCID
- PMC13243919
- DOI
- 10.5713/ab.250773
Abstract (original English)
Objective This study investigates the molecular mechanisms by which the deubiquitinase MYSM1 regulates the proliferation and differentiation of bovine skeletal muscle satellite cells (BSMSCs), thereby providing new theoretical insights into the regulation of muscle growth and development in beef cattle. Methods An in vitro model of BSMSCs was established to investigate the role of MYSM1. The expression pattern of MYSM1 during cell proliferation and differentiation was analyzed using quantitative real-time polymerase chain reaction (qRT-PCR). MYSM1 knockdown models were generated, and the expression of proliferation markers PAX7 and Ki67, as well as differentiation markers MYHC and MYOG, were examined by qRT-PCR and Western blotting. Analysis of protein-protein interactions identified BRG1 as a potential MYSM1 interactor, and its function was subsequently evaluated. Downstream signaling activity was evaluated by examining phosphorylation changes in key components of the AKT/mTOR pathway. In addition, global histone ubiquitination H2AK119ub1 and the histone methylation markers H3K4me3 and H3K27me3 were analyzed following MYSM1 knockdown. Results MYSM1 expression was dynamically regulated, exhibiting significant upregulation during differentiation, reaching its highest at days 2-3 (p Conclusion MYSM1 promotes the proliferation and differentiation of BSMSCs through BRG1-mediated ep
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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