A new method for Schwann-like cell differentiation of adipose derived stem cells.
Liu Y., Zhang Z., Qin Y., Wu H., Lv Q., Chen X.
Animal Study, published in Neurosci Lett (2013) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Neurosci Lett (2013)
- Country
- Ireland
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 23880021
- DOI
- 10.1016/j.neulet.2013.07.012
- Citations
- 43
Abstract (original English)
Peripheral nerve repair can be enhanced by Schwann cell transplantation, but the clinical application of this procedure is limited by donor site morbidity and the inability to quickly generate a sufficient number of cells. Thus, alternative cell systems for the generation of Schwann cells are desired. Schwann-like cell induced from adipose-derived stem cells (ADSCs) may be one of the ideal alternative cell systems for Schwann cell generation. Although co-culture with Schwann cells or chemicals combined with a mixture of glial growth factors are often utilized for Schwann cell-like differentiation of ADSCs, these methods are usually complicated or expensive. In this experiment, the rat sciatic nerve was cut, and then soaked in culture medium for two days. The treated culture medium was used as an induction agent after filtering. The obtained ADSCs were incubated with the above induction culture medium for five days. Then, expression of the typical Schwann cell markers, S-100 and GFAP proteins was determined by immunocytochemical staining and Western blotting. The results showed that almost all of the treated ADSCs displayed a spindle shape like morphology after being incubated with induction culture medium for 24h and expressed S-100 and GFAP proteins after five days. All of these characteristics of differentiated rat ADSCs were similar to genuine Schwann cells. Thus, this new m
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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