Novel method for the isolation of adipose stem cells (ASCs).
Rada T., Reis RL., Gomes ME.
Animal Study, published in J Tissue Eng Regen Med (2009) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- J Tissue Eng Regen Med (2009)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 19111010
- DOI
- 10.1002/term.141
- Citations
- 34
Abstract (original English)
Adipose stem cells (ASCs) represent a cell population with great potential for tissue engineering applications. Several articles have been published showing the proliferation and differentiation potential, the markers and the wide range of potential applications of these cells. In the majority of these studies the ASCs are isolated using a basic enzymatic procedure, which results in a quite heterogeneous cell population that compromises their proliferation and differentiation. This paper reports the development and optimization of a new isolation/purification method that allows populations of ASCs to be obtained, which significantly reduces (and eventually eliminates) the 'contamination' of other cell types. This method is based on the use of immunomagnetic beads coated with specific antibodies. The first part of the study described here analysed the expression of marker genes for stem cells and the colony-forming unit (CFU) capacity of the cells isolated, while the second part is dedicated to the osteogenic differentiation potential of the isolated cells. The results showed that, using the isolation method based on immunomagnetic beads, it was possible to obtain ASCs and also underline the existence of several subpopulations of stem cells in the adipose tissue.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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