Nuclear factor 1 is essential for the expression of stearoyl-CoA desaturase 1 gene during preadipocyte differentiation.
Singh MV., Ntambi JM.
Animal Study, published in Biochim Biophys Acta (1998) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Biochim Biophys Acta (1998)
- Country
- Netherlands
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 9689914
- DOI
- 10.1016/s0167-4781(98)00037-2
Abstract (original English)
Stearoyl CoA desaturase gene 1 (SCD1) is a delta 9 desaturase that is transcriptionally activated during the differentiation of 3T3-L1 preadipocytes into adipocytes. We have demonstrated that a SCD1/BP region in SCD1 proximal promoter (-114 to -86 bp) is essential for the transcriptional activation of this gene during differentiation. Mutation in this region abolished the basal activity of the proximal promoter of SCD1, and also failed to induce transcription in response to the differentiation cocktail in transfected cells. The SCD1/BP region contains a TGGCA sequence at -90 bp from the transcription start site. Using competitor oligonucleotides and nuclear factor 1 (NF1)-specific antibodies in gel shift assays, we have shown that in preadipocytes, a NF1 protein binds to this TGGCA sequence. On MDI-induced differentiation of preadipocyte into adipocyte, an additional DNA-protein complex appeared. The appearance of a new NF1 complex is related to the differentiation-specific transcriptional activation of the SCD1 gene. This is the first report to show a differentiation-related change in NF1 protein binding during preadipocyte differentiation.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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