Numerical simulation analysis of flow field and fabrication of cells-osteochondral scaffold constructs in a spinner flask bioreactor.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

With the vigorous development of bone/cartilage tissue engineering research, the construction system for in vitro preparation of tissue engineered osteochondral repair substitutes is undergoing a transformation from static culture mode to 3D dynamic culture mode. However, for dynamic culture mode, many problems such as the selection of cultivation environment and the optimization of condition parameters need to be solved. In this study, computational fluid dynamics (CFD) was used to simulate and predict the stress conditions of adipose derived stem cells-chitosan 7 /gelatin 3 /Nano-hydroxyapatite (ADSCs-Cs 7 /Gel 3 /nHAP) structures and ADSCs-bone-derived scaffold structures, as well as the flow field distribution in spinner flask (SF) at different rotational speeds. Finally, the appropriate operating conditions of SF were optimized. The simulation results showed that SF generated two fluid cycles bounded by the bottom edge of the stirring paddle in the entire fluid flow region, with a fluid circulation region exhibiting a relatively static flow field distribution (compared with the first two cycles) directly below the stirring axis. There was a moderate dynamic pressure and speed under the stirring paddle, making this area the most suitable for fixing the cell-scaffold constructs. Under different rotational speed conditions, the dynamic pressure and fluid shear force of the co

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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