SVF
Level D· Preclinical EvidenceLaboratory Study

Oil Droplet in Apoptotic Uniocular Adipocyte: A Double-Edged Sword in Determining Macrophage Phenotype and its Implications on Fat Grafting.

Lei C., Wang S., Huang T., Xu N., Ye J., Wang M.

Laboratory Study on Face & Skin, published in Aesthetic Plast Surg (2026) — summary generated from the PubMed abstract.

This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.

Study type
Laboratory Study
Journal
Aesthetic Plast Surg (2026)
Country
United States
Reported sample size
—
PMID
41269253
DOI
10.1007/s00266-025-05351-4

Abstract (original English)

Fat grafting procedure is increasingly popular. However, unstable retention rate hindered its further application. Research primarily focuses on the revascularization of grafted fat. The membrane mature adipocyte aggregate cultures method was used to culture uniocular adipocytes in vitro. Staurosporine was used to induce apoptosis in uniocular adipocytes, multi-ocular adipocytes, and adipose-derived stem cells (ASCs). The apoptotic uniocular adipocytes were examined by scanning electron microscopy, and methanol was used to stimulate fusion of apoptotic adipocytes. Immunofluorescence staining and Western blotting were used to identify macrophage phenotypes after co-culture with apoptotic uniocular adipocytes and fused oil droplets. Uniocular adipocytes maintained their morphology even after apoptosis induction, while multi-ocular adipocytes and ASCs cracked and released cellular components. Methanol induced fusion of apoptotic uniocular adipocytes, and the fused large oil droplets attracted multiple cell debris, as indicated by DAPI/PI staining. Co-culture with apoptotic uniocular adipocytes induced the M2 polarization, whereas co-culture with fused oil droplets induced M1 polarization. This study demonstrated that the surface tension of uniocular adipocytes preserves basic cellular morphology and promotes M2 polarization. In contrast, in unstable culture environment (e.g., meth

What this study does not prove

  • • This study does not prove SVF is an approved treatment or a replacement for standard care.
  • • This is preclinical work; animal or laboratory results cannot be applied to humans.

Evidence level

Evidence comes from animal or laboratory studies and has not been confirmed in humans.

How we grade evidence
ApoptosisAdipocytesMacrophagesAdipose TissueHumansCells, CulturedPhenotypeCoculture TechniquesMicroscopy, Electron, Scanning
Face & Skin
View on PubMedDOI

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