Osteogenic differentiation of human adipose-derived stem cells in 3D conditions - comparison of spheroids and polystyrene scaffolds.
Rumiński S., Kalaszczyńska I., Długosz A., Lewandowska-Szumieł M.
Laboratory Study, published in Eur Cell Mater (2019) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Eur Cell Mater (2019)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 31099888
- DOI
- 10.22203/eCM.v037a23
- Citations
- 25
Abstract (original English)
Expansion and differentiation of adipose-derived stem cells (ADSCs) in vitro are routinely performed in two-dimensional (2D) environments. The study hypothesis was that the utilisation of three-dimensional (3D) culture conditions, mimicking the natural stem cell niche, might increase the osteogenic commitment of ADSCs. Therefore, human ADSCs were seeded in 3D culture systems lacking bioactive material components: spheroids and polystyrene scaffolds. ALP activity, a marker of early osteogenesis, was higher in ADSC spheroids and ADSC seeded on polystyrene scaffolds as compared to 2D cultures. Furthermore, the expression of the osteoblast marker genes Runt-related transcription factor 2 (RUNX2), osterix and integrin binding sialoprotein (IBSP) was significantly up-regulated in spheroids as compared to polystyrene scaffolds and 2D culture. Elevated levels of RUNX2 and IBSP in spheroids were confirmed at the protein level by Western blot and immunofluorescence, respectively. Bone mineral production was lower in spheroids than in polystyrene scaffolds and 2D culture at day 14. Curiously, adipocyte differentiation was downregulated in spheroids as compared to 2D-culture. Finally, to induce late differentiation events, cells were dissociated from spheroids after a 7 d osteogenic pre-differentiation culture and replated in 2D culture in osteoblast maturation medium. After a subsequent 1
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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