[Overexpression of core binding factor alpha1 enhancing osteoblastic differentiation in adipose-derived stem cells].
Zhang X., Yang M., Lin L., Ao YF.
Animal Study, published in Zhonghua Wai Ke Za Zhi (2008) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Zhonghua Wai Ke Za Zhi (2008)
- Country
- China
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 18844059
Abstract (original English)
Objective To determine whether mouse core binding factor alpha1 (Cbfalpha1) overexpression enhancing osteoblastic differentiation in adipose-derived stem cells (ADSCs). Methods ADSCs were harvested from SD rats and transduced with the recombinant adenovirus carrying Cbfalpha1 gene. Untransduced cells and cells transduced with adenovirus carrying the enhanced green fluorescence protein (Ad-EGFP) gene served as controls. Cbfalpha1 expression was assessed by immunofluorescence. Alkaline phosphatase (ALP) activity was assayed in 7 and 10 d. Results Overexpression of Runx2 inhibited adipogenesis, as demonstrated by suppression of LPL expression. Moreover, ADSCs transduced with Ad-Runx2 underwent rapid and marked osteoblast differentiation as determined by osteoblastic gene expression, alkaline phosphatase activity. Conclusions Cbfalpha1 overexpression enhances osteoblastic differentiation in adipose-derived stem cells.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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